T4 Polynucleotide Kinase (3 phosphatase minus) from New England Biolabs

Product Specs
ItemT4 Polynucleotide Kinase (3 phosphatase minus)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0236L
Quantity1000 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [³²P] in 30 minutes at 37°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seqSTAR ProtocolsSeptember 1, 2023Isabel Cristina Vélez-Bermúdez, Shu-Jen Chou, Ai-Ping Chen, Wen-Dar Lin, Wolfgang SchmidtRNaseZap RNase Decontamination Solution,Invitrogen,Cat#AM9780 T4 Polynucleotide kinase reaction buffer (10× T4 PNK buffer),New England Biolabs,Cat#M0236S T4 Polynucleotide kinase (10 U/μL),New England Biolabs,Cat#M0201S Linear acrylamide (5 mg/mL) (1 mL tube

(2) DDM1-mediated R-loop resolution and H2A.Z exclusion facilitates heterochromatin formation in ArabidopsisSci AdvAugust 11, 2023Jincong Zhou, Xue Lei, Sarfraz Shafiq, Weifeng Zhang, Qin Li, Kuan Li, Jiafu Zhu, Zhicheng Dong, Xin-Jian He, Qianwen SunT4 polynucleotide kinase (T4 PNK) reaction mixture containing 75 μl of T4 PNK buffer, 10 μl of T4 PNK enzymes (NEB, M0236), and 15 μl of 10 mM ATP and incubated at 37°C for 10 min on a Thermomixer (1400 rpm). After that, the

(3) H3K4me3 regulates RNA polymerase II promoter-proximal pause-releaseNatureMarch 9, 2023Hua Wang, Zheng Fan, Pavel V. Shliaha, Matthew Miele, Ronald C. Hendrickson, Xuejun Jiang, Kristian Helinreaction mix containing 1× PNK buffer, 0.1% (v/v) Tween-20, 1 mM ATP and T4 PNK 3′ phosphatase minus (NEB, M0236L) at 37 °C for 10 min. We eluted the RNA by adding 350 μl of buffer RLT plus and 1 ng of fragmented Drosophila

(4) Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infectionCell reportsMarch 9, 2023Fossat N, Lundsgaard EA, Costa R, Rivera-Rangel LR, Nielsen L, Mikkelsen LS, Ramirez S, Bukh J, Scheel TKH.(phosphorylation-3′-minus buffer 1X, ATP 1 mM, T4 polynucleotide kinase 3′ phosphatase minus 40 U [New England Biolabs M0236L], RNasin Plus 80 U) and incubated for 20 min at 37°C with intermittent shaking for 15 s every 2 min

(5) Chemoproteomic discovery of a human RNA ligaseNature CommunicationsFebruary 15, 2023Yizhi Yuan, Florian M. Stumpf, Lisa A. Schlor, Olivia P. Schmidt, Philip Saumer, Luisa B. Huber, Matthias Frese, Eva Höllmüller, Martin Scheffner, Florian Stengel, et al.bearing 2′,3′-cPO4 or 2′-PO4-3′-OH on the 3′-ends, T4 PNK 3’ phosphatase minus (New England BioLabs, M0236S) was used. General procedures of RNA ligation assays As for general procedure of RNA ligation with

(6) Cultural and demic co-diffusion of Tubo Empire on Tibetan PlateauiScienceDecember 22, 2022Kongyang Zhu, Panxin Du, Jiyuan Li, Jianlin Zhang, Xiaojun Hu, Hailiang Meng, Liang Chen, Boyan Zhou, Xiaomin Yang, Jianxue Xiong, et al.,Sigma Aldrich,P9416 ATP,Thermo,R0441 dNTP Mix,Thermo,R1121 T4 DNA Ligase,Thermo,EL0011 T4 Polynucleotide Kinase,New England Biolabs,M0236L T4 DNA Polymerase,New England Biolabs,M0203L Bst enzyme,New England Biolabs,M0275S Q5 High-Fidelity DNA Polymerase

(7) Coelenterazine sulfotransferase from Renilla muelleriPLOS ONEOctober 17, 2022George Tzertzinis, Brenda Baker, Jack Benner, Elizabeth Brown, Ivan R. CorrÃªa, Laurence Ettwiller, Colleen McClung, Ira Schildkrautrelative light units per 1 second (RLU/s). PAP assay T4 polynucleotide kinase (3’-phosphatase minus, M0236) was obtained from New England Biolabs (NEB). 3’-phosphoadenosine 5’-phosphate and adenosine 3’-monophosphate

(8) Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activityMolecular CellMarch 17, 2022Connor A. Tsuchida et al.DNase I,Promega,Cat#M6101 "ATP, [g-32P]- 3000Ci/mmol",Perkin Elmer,Cat#BLU002A001MC T4 PNK,NEB,Cat#M0236S Proteinase K,Sangon Biotech,Cat#A600451-0050 BbsI-HF,NEB,Cat#R3539L AgeI,NEB,Cat#R3552L BamHI,NEB,Cat#R0136L KpnI,NEB,Cat#R3142L PciI,NEB,Cat#R0655L "Dulbecco’s

(9) Phospho-RNA sequencing with circAID-p-seqNucleic Acids ResearchFebruary 28, 2022Alessia Del Piano et al.RNA fragments bearing a 3′P were subjected to 5′ phosphorylation with T4 PNK 3′ minus (NEB, cat no. M0236S), according to manufacturer's instructions. RNA fragments were purified using a RNA Clean & Concentrator™-5

(10) A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicingMolecular cancerJanuary 15, 2022El Fatimy R, Zhang Y, Deforzh E, Ramadas M, Saravanan H, Wei Z, Rabinovsky R, Teplyuk NM, Uhlmann EJ, Krichevsky AM.beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3′- phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB, M0204S). The beads were further washed

(11) Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycleNature CommunicationsDecember 1, 2021Esther Griesbach, Margarita Schlackow, William F. Marzluff, Nick J. Proudfootwas end-labelled with 32P-γ-ATP (PerkinElmer, #NEG502Z250UC) using T4 polynucleotyde kinase (NEB, #M0236S). The labelled probes (dsDNA and ssDNA) were purified using Illustra Microspin G-25 columns (GE Healthcare

(12) Ancient genome analyses shed light on kinship organization and mating practice of Late Neolithic society in ChinaiScienceNovember 19, 2021Chao Ning, Fan Zhang, Yanpeng Cao, Ling Qin, Mark J. Hudson, Shizhu Gao, Pengcheng Ma, Wei Li, Shuzheng Zhu, Chunxia Li, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, T4 Polynucleotide Kinase,New England Biolabs,M0236L T4 DNA Polymerase,New England Biolabs,M0203L Bst DNA Polymerase,New England Biolabs,M0275S Q5 High-Fidelity DNA Polymerase

(13) Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesionCellJuly 8, 2021Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM.R0734S) digested vectors by a 1:2 mixture of T4 ligase (NEB, M0202L) and T4 polynucleotide kinase (NEB, M0236L) in T4 ligase buffer. The ligation mix was transformed using NEB stable competent cells (NEB, C3040)

(14) eNEMAL, an enhancer RNA transcribed from a distal MALAT1 enhancer, promotes NEAT1 long isoform expressionPLOS ONEMay 21, 2021Joshua K. Stone, Lana Vukadin, Eun-Young Erin Ahn(5’-ACGCGTGCGCGTTTAAA CGCGCTCGCGAGC- 3’) was 5’ phosphorylated by T4 Polynucleotide Kinase (T4 PNK, 3’ phosphatase minus, NEB #M0236S) per protocol instructions. Next, T4 RNA Ligase 1 (NEB #M0204S) was used to ligate the PNK phosphorylated

(15) The DNA methyltransferase DNMT3A contributes to autophagy long-term memoryAutophagyMay 1, 2021González-Rodríguez P, Cheray M, Füllgrabe J, Salli M, Engskog-Vlachos P, Keane L, Cunha V, Lupa A, Li W, Ma Q, et al.[Invitrogen, 10,977–035], 5.2 μl T4 PNK buffer, 1 μl SUPERase In and 1 μl T4 PNK [New England Biolabs/NEB, M0236]) and incubated at 37°C for 1 h. The RNA was extracted and precipitated using acidic phenol-chloroform.

(16) Real-time observation of Cas9 postcatalytic domain motionsProceedings of the National Academy of SciencesJanuary 14, 2021Yanbo Wang, John Mallon, Haobo Wang, Digvijay Singh, Myung Hyun Jo, Boyang Hua, Scott Bailey, Taekjip Hadetails. Radio-Labeled Cleavage Assays. Radioactive ATP γ-32P (PerkinElmer) along with T4 Polynucleotide Kinase (NEB, M0236S) was used to label the 5′ end of the target strand and/or nontarget strand. Cas9 and guide RNA were

(17) CRISPR-Cas “Non-Target” Sites Inhibit On-Target Cutting RatesThe CRISPR JournalDecember 21, 2020Eirik A. Moreb, Mitchell Hutmacher, Michael D. LynchHot-Start High Fidelity Master Mix (NEB, M0493S). Reagents for the KLD reactions came from NEB: T4 PNK (M0236S), T4 DNA ligase (M0202S), and DpnI (R0176S). All primers and sequences used in construction of these

(18) Interplay of pericentromeric genome organization and chromatin landscape regulates the expression of Drosophila melanogaster heterochromatic genesEpigenetics & ChromatinDecember 1, 2020Parna Saha, Divya Tej Sowpati, Mamilla Soujanya, Ishanee Srivastava, Rakesh Kumar Mishra50 µM final concentration. The reverse primers were phosphorylated using Polynucleotide kinase (NEB M0236S). Conversion of 3C libraries to 5C libraries 3C and control libraries were separately mixed with salmon

(19) Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of TelomeresCell reportsAugust 4, 2020Segura-Bayona S, Villamor-Payà M, Attolini CS, Koenig LM, Sanchiz-Calvo M, Boulton SJ, Stracker TH.M-PER Mammalian Protein Extraction Reagent,Thermo Fisher Scientific,Cat# 78503 T4 Polynucleotide Kinase,NEB,Cat# M0236S MinElute PCR Purification Kit,QIAGEN,Cat# 28004 PureLink Quick Gel Extraction & PCR Purification Combo

(20) Hierarchy in Hfq Chaperon Occupancy of Small RNA Targets Plays a Major Role in Their RegulationCell ReportsMarch 3, 2020Raya Faigenbaum-Romm, Avichai Reich, Yair E. Gatt, Meshi Barsheshet, Liron Argaman, Hanah MargalitRNase A/T1 mix,ThermoFischer Scientific,Cat #: EN0551 T4 Polynucleotide Kinase,New England Biolabs,Cat #: M0236L "T4 RNA ligase 1, High Concentration",New England Biolabs,Cat #: M0437M RNAClean XP,Beckman Coulter,Cat

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